Output of primer pairs: Output files include a table of primer sequences to be ordered, a BED file of primer locations that can be used subsequently for primer trimming and a diagram of the primer Coverslips were incubated with a mouse monoclonal antibody against syt (mAb48, diluted 1:1,000, Developmental Studies Hybridoma Bank) 51 and, to visualize nerve terminals, a polyclonal guinea pig .
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